Objective A high-density genetic map of ‘Baijiguan’ Tea and ‘Fuyun No. 6’ Tea was constructed to facilitate the increasingly popular studies concerning the regulatory mechanism of albino traits in tea plants.
Method Populations of genetically isolated F1 generation of Baijiguan (♀) and Fuyun No. 6 (♂) were created. SNP molecular markers were developed by SLAF-seq technology. HighMap was the software applied to establish high-density linkage maps.
Result A reference tea genome was used for electronic digestion prediction to select the HaeIII scheme. In the length of 314 bp to 364 bp, 230000 SLAF tags were predicted. With basically uniform distribution of SLAF tags on each chromosome, the enzyme digestion scheme was deemed feasible. The library construction had an accuracy showing a normal paired-end mapped efficiency of 85.98% and enzyme digestion efficiency of 91.82% on the control data. A total of 828448417 reads (165.67 Gb) sequencing data were obtained with an average Q30 of 93.94% and GC content of 43.67% indicating a normal GC distribution on the samples. Of the 5975507 SNP markers obtained, 643601 were successfully typed, 500988 used for the map construction, and 7967 finally mapped. The resulting high-density genetic map had a spacing of 2754.59 cM, a marker integrity of 99.84%, and 15 linkage groups.
Conclusion A high-density genetic map containing 15 linkage groups were constructed using the genetically isolated populations of F1 generation Baijiguan and Fuyun No. 6 teas.
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