High-throughput Unveiling ofMicrosatellite Markers Using Ectropisgrisescens Transcriptome

The tea Geometrid moth Ectropis grisescens is themost devastating insect pest of tea plants, which widely distributed in all tea-growingareas in China. For high-throughput excavation single sequence repeat (SSR)markers from E. grisescens, thesoftware MISA was employed to filter 66468 unigenes obtained from thetranscriptome of E. grisescens forSSR. The results showed that 18339 SSR loci were identified, and the frequencyof occurrences for SSRs was 27.59%. These SSR loci were distributed in 13465unigenes with a distribution frequency of 20.26%. Most repeat type was mononucleotide motifs,accounting for 68.14% of all SSRs. There were 84 kinds ofrepeat motifs in the E. grisescens transcriptome, in which A/Twas the dominant type of mononucleotide repeat motifs, accounting for 65.47% of all SSRs. The number of tenrepeat SSRs was the most, which were 6822(37.20%), followed by 11 repeats2526 (13.77%). Our study indicated that there were a large number ofSSRs in transcriptome of E. grisescens with high frequency andabundant motif types. All of this information would lay a foundation for thefurther study of population genetic diversity, population origin and evolution,and integrated pest management of E. grisescens.