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茶树捕光色素蛋白复合体CsLhcb1基因的鉴定及干旱胁迫响应分析

Identification and Drought Stress Response of CsLhcb1 in Tea Plants

    摘要
  • 摘要:
    目的 捕光色素蛋白复合体是高等植物中能够捕获光能，并把能量快速传递至反应中心从而引起光化学反应的一类色素蛋白复合体，在光合作用中发挥着不可代替的作用。采用基因克隆、生物信息学分析和基因表达等方法探究干旱胁迫下CsLhcb1在茶树中的作用，为进一步揭示茶树CsLhcb1基因的功能提供理论依据。
    方法 以茶树品种‘龙井43’‘舒茶早’和‘白叶一号’为试验材料，利用PEG-6000（20%）模拟干旱胁迫环境，通过基因克隆、生物信息学以及实时荧光定量的方法，检测CsLhcb1的结构和特征及其在干旱胁迫响应。
    结果 从‘龙井43’的cDNA中克隆获得CsLhcb1基因，序列分析表明，该基因全长为810 bp，共编码269个氨基酸；该蛋白的分子量为29065.36 Da。荧光定量结果显示，干旱胁迫，茶树品种‘龙井43’中的CsLhcb1基因相对表达量在1 h时达到最高值，之后逐渐下降；在‘舒茶早’中该基因相对表达量也在1 h时达到最高值，之后下降，24 h又上升；在‘白叶一号’中该基因的相对表达量在6 h时达到最高值，之后下降，在1h时‘舒茶早’的表达量分别为‘龙井43’和‘白叶一号’的1.49、1.6倍；在24 h时‘舒茶早’的表达量分别为‘龙井43’和‘白叶一号’的9.56、5.73倍。
    结论 干旱胁迫影响了捕光色素蛋白复合体基因CsLhcb1的表达，且在不同的茶树品种中表达量有差异。

     

    Abstract:
    Objective Role of CsLhcb1 associated with the chlorophyll-protein complex which captures light energy to initiate the photochemical reaction in tea plants was investigated, and the genetic expression under drought stress studied to understand the function it plays.
    Method Gene cloning, bioinformatic, and real-time fluorescence quantitative methods were applied to reveal the structure and characteristics of CsLhcb1. Gene expressions under a simulated drought with 20% PEG-6000 on tea cultivars Longjing 43, Shuchazao, and Baiye No. 1 were conducted.
    Result CsLhcb1 was cloned from the cDNA of Longjing 43 to show a full length of 810 bp encoded 269 amino acids with a molecular weight of 29,065.36 Da. Under the simulated drought condition, its expression peaked in 1h followed by a gradual decline. So did that in Shuzhazao but rose after 24 h, whereas in Baiye No. 1, it did not reach the maximum for 6 h before a decline. In 1 h after the imposed stress, the expression in Shuchazao was 1.49x of that in Longjing 43 and 1.6x of that in Baiye No. 1, while in 24 h, it was 9.56x in Longjing 43 and 5.73x of that in Baiye No. 1.
    Conclusion Drought stress significantly affected the expression in varying degrees of CsLhcb1 of different tea cultivars.

     

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