高级搜索
詹重清. QuEChERS-气相色谱-三重四极杆串联质谱法测定茶叶中5种农药残留［J］. 茶叶学报，2023，64（6）：85−91. DOI: 10.20045/j.cnki.issn.2096-0220.2023.06.009
引用本文: 詹重清. QuEChERS-气相色谱-三重四极杆串联质谱法测定茶叶中5种农药残留［J］. 茶叶学报，2023，64（6）：85−91. DOI: 10.20045/j.cnki.issn.2096-0220.2023.06.009
shu
ZHAN Chong-qing. Determination of Pesticide Residues in Tea by QuEChERS Extraction and GC-Triple Quadrupole Tandem MS[J]. ACTA TEA SINICA, 2023, 64(6): 85-91. DOI: 10.20045/j.cnki.issn.2096-0220.2023.06.009
Citation: ZHAN Chong-qing. Determination of Pesticide Residues in Tea by QuEChERS Extraction and GC-Triple Quadrupole Tandem MS[J]. ACTA TEA SINICA, 2023, 64(6): 85-91. DOI: 10.20045/j.cnki.issn.2096-0220.2023.06.009
shu

QuEChERS-气相色谱-三重四极杆串联质谱法测定茶叶中5种农药残留

Determination of Pesticide Residues in Tea by QuEChERS Extraction and GC-Triple Quadrupole Tandem MS

    摘要
  • 摘要:
    目的 建立分散固相萃取技术（QuEChERS）结合气相色谱-三重四极杆串联质谱法（GC-MS/MS）同时测定茶叶中烯虫乙酯、烯虫炔酯、格螨酯、氟除草醚和草枯醚5种农药的检测方法。
    方法 磨碎茶样首先用纯水浸泡30 min，再加入乙腈超声提取，QuEChERS法净化，用GC-MS/MS外标法定量。
    结果 该方法具有较高的灵敏度，烯虫乙酯、格螨酯、氟除草醚和草枯醚的定量限为0.003 mg·kg−1，烯虫炔酯的定量限为0.01 mg·kg−1；5种农药在0.004～0.40 μg·mL−1浓度内的二次拟合相关系数≥0.998，平均回收率为75.4%～108.4%，相对标准偏差为3.5%～7.7%。
    结论 该方法简便、灵敏度高、成本低，适用于茶叶中烯虫乙酯、烯虫炔酯、格螨酯、氟除草醚和草枯醚的测定。

     

    Abstract:
    Objective A method using QuEChERS extraction followed by gas chromatography-triple quadrupole tandem mass spectrometry (GC-MS/MS) to detect residues of five pesticides in tea was developed.
    Method Ground tea samples were soaked in distilled water for 30 min and extracted with acetonitrile under ultrasonication. The extract was purified by QuEChERS method and analyzed by GC-MS/MS with external standards.
    Result A high sensitivity of the assay was demonstrated by the limits of quantitation on hydroprene, genite, fluoronitrofen, and chlornitrofen at 0.003 mg·kg−1 and on kinoprene at 0.01 mg·kg−1 with a quadratic fitting coefficients greater than and equal to 0.998, respectively, in the concentration range of 0.004~0.40 μg·mL−1. The average rate of assay recovery was 75.4~108.4% with a relative standard deviation of 3.5~7.7%.
    Conclusion The newly developed method was simple, sensitive, and economic in operation. It was deemed suitable for the determination of hydroprene, kinoprene, genite, fluoronitrofen, and chlornitrofen residues in tea.

     

    • HTML全文
    • 参考文献(23)
    • 相关文章
    • 施引文献
  • 资源附件(0)

/

返回文章
返回