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QuEChERS-GC-MS法快速检测茶叶中的芳樟醇和alpha-紫罗兰酮

A Rapid QuEChERS-GC-MS Assay for Linalool and Alpha-ionone Determinations in Tea

  • 摘要:
    目的 为完善茶叶中香气物质的检测方法,针对芳樟醇与alpha-紫罗兰酮两种香气物质建立气相色谱-质谱联用法(Gas Chromatography-Mass Spectrometry,GC-MS)快速同时检测的分析方法,以期为茶叶中香气物质的检测提供新思路和参考。
    方法 茶叶样品经乙腈提取后,用QuEChERS净化管(内含PSA 200 mg、GCB 200 mg、无水硫酸钠400 mg)净化茶叶上清液,采用GC-MS选择离子监测模式进行测定,外标法定量。
    结果 芳樟醇和alpha-紫罗兰酮在5.0~2000.0 μg·L−1内线性良好,线性相关系数(R2)均大于0.999;方法的检出限为分别为0.020 mg·kg−1和0.004 mg·kg−1,定量限分别为0.080 mg·kg−1和0.010 mg·kg−1。使用该方法在空白茶叶样品中分别添加3种不同加标浓度(5.0、10.0、1000.0 μg·L−1)的混合标准溶液,测得平均回收率为88.23%~106.11%,相对标准偏差(RSD)为1.98%~3.25%(n=6)。
    结论 该方法操作快速便捷,运行时间仅需16.8 min,重现性和精确度高、灵敏度好,适用于茶叶中芳樟醇、alpha-紫罗兰酮的快速检测,能够为相关机构对茶叶中香气物质的检测提供技术支持。

     

    Abstract:
    Objective An assay to detect the aromatic linalool and alpha-ionone in tea applying gas chromatography-mass spectrometry (GC-MS) was developed.
    Method Tea samples were firstly extracted by acetonitrile to collect the supernatant for the QuEChERS purification on a column containing 200 mg of PSA, 200 mg of CB, and 400 mg of anhydrous sodium sulfate prior to GC-MS analysis. Under the ion monitoring mode (SIM), aromatic compounds were quantifiably determined with external standards.
    Result The assay detection limit on linalool was 0.020 mg·kg−1 and that on alpha-ionone 0.004 mg·kg−1; while the quantification limit was 0.080 mg·kg−1 on linalool and 0.010 mg·kg−1 on alpha-ionone. The measurement curves on the two aromatherapy substances were linear in concentrations of 5.0-2000.0 μg·L−1 with a greater than 0.999 correlation coefficient (R2). On the blank control sample of tea spiked with 5.0, 10.0, and 1000.0 μg·L−1 of standard linalool and alpha-ionone, the assay showed an average recovery of 88.23%-106.11% with a relative standard deviation (RSD) of 1.98%-3.25% (n=6).
    Conclusion The newly developed method was rapid with a running time of only 16.8 m and highly reproducible, accurate, and sensitive results. It was deemed applicable for rapid detection of linalool and alpha-ionone in tea.

     

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