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茶树品种‘白鸡冠’与‘福云6号’遗传连锁图谱构建

Genetic Map of ‘Baijiguan’ and ‘Fuyun No. 6’ Teas

    摘要
  • 摘要:
    目的 近年来对茶树白化性状调控机理的研究逐渐增多，但到目前为止白化性状的基因调控机制仍然未知。通过构建‘白鸡冠’与‘福云6号’高密度遗传连锁图谱，为后续白鸡冠叶色基因定位奠定基础。
    方法 创建白鸡冠（♀）与福云6号（♂）F1代遗传分离群体，采用SLAF-seq技术和HighMap软件开发SNP分子标记，构建高密度遗传连锁图。
    结果 用茶树参考基因组作电子酶切预测，选用HaeIII酶切方案。SLAF标签长度选择314～364 bp，预测到230000个SLAF标签。SLAF标签在基因组各染色体上分布基本均匀，酶切方案可行。建库准确性评估发现，对照样数据的双端比对效率为85.98%，酶切效率为91.82%，说明SLAF建库正常。本实验共获得828448417条 reads（165.67 Gb）测序数据，测序平均Q30为93.94%，平均GC含量为43.67%，样本GC分布正常。获得SNP标记5975507个，成功分型的有643601个，用于遗传图谱构建的标记有500988个，最终上图标记有7967个，总图距为2754.59 cM，上图标记完整度为99.84%，共构建出15个高密度遗传连锁群。
    结论 利用白鸡冠与福云6号F1代遗传分离群体构建出含15个连锁群的高密度遗传连锁图谱。

     

    Abstract:
    Objective A high-density genetic map of ‘Baijiguan’ Tea and ‘Fuyun No. 6’ Tea was constructed to facilitate the increasingly popular studies concerning the regulatory mechanism of albino traits in tea plants.
    Method Populations of genetically isolated F1 generation of Baijiguan (♀) and Fuyun No. 6 (♂) were created. SNP molecular markers were developed by SLAF-seq technology. HighMap was the software applied to establish high-density linkage maps.
    Result A reference tea genome was used for electronic digestion prediction to select the HaeIII scheme. In the length of 314 bp to 364 bp, 230000 SLAF tags were predicted. With basically uniform distribution of SLAF tags on each chromosome, the enzyme digestion scheme was deemed feasible. The library construction had an accuracy showing a normal paired-end mapped efficiency of 85.98% and enzyme digestion efficiency of 91.82% on the control data. A total of 828448417 reads (165.67 Gb) sequencing data were obtained with an average Q30 of 93.94% and GC content of 43.67% indicating a normal GC distribution on the samples. Of the 5975507 SNP markers obtained, 643601 were successfully typed, 500988 used for the map construction, and 7967 finally mapped. The resulting high-density genetic map had a spacing of 2754.59 cM, a marker integrity of 99.84%, and 15 linkage groups.
    Conclusion A high-density genetic map containing 15 linkage groups were constructed using the genetically isolated populations of F1 generation Baijiguan and Fuyun No. 6 teas.

     

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