Abstract:
Objective Ectropis grisescens Warren, a common leaf-eating pest in tea plantations, was widely distributed in various tea producing cities in Fujian Province and caused certain economic losses. To develop and design primers for microsatellite loci of grey tea inchworm, and to study the genetic differences of different geographical populations in Fujian Province, in order to understand their population occurrence rules and provide references for the development of sustainable prevention and control technologies.
Method MISA was used to search the genome and transcriptome sequences of E. grisescens Warren for loci distribution. Primer 3 and TBtools were employed to design the primers for the simulated amplification. The three-primer fluorescence labeling method was applied to obtain the pairs of polymorphic microsatellite loci for differentiating the specimens of 4 population groups collected in the province.
Result The repeat types of microsatellite loci in E. grisescens were numerous, and the repeat motifs showed apparent AT bias. The 4 population groups significantly deviated from the Hardy-Weinberg equilibrium on part of the 5 identified pairs of polymorphic loci.
Conclusion The genetic deviation in the E. grisescens population at Quanzhou was the greatest and that at Ningde significant among the 4 groups. There was no significant difference between those at Sanming and Nanping.
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