Abstract: Waste from shiitake cultivated on substratecontaining trigs and leaves form tea bushes was used to replace chemicalfertilizers at a tea plantation for this study. Nitrogen (N) replacements forthe experimentation were designed to be 100, 80, 60, 40, 20, and 0%. The yieldof the plants and biochemical composition of the leaves from the tea bushesgrown under various fertilization treatments for 3 years were determined. Theresulting output on fresh tea leaves on the plants decreased as the percentageof the mushroom waste to replace N fertilizer for fertilization increased. Theyield of 6196.80 kg?hm^-2 under treatment1 replacement reduced 10.41% of that of CK. For the other replacements, thereductions on leaf yield ranged from as low as 0.48% to the highest at 10.41%.Significantly different from the other treatments (P <0.05),treatment 3 produced the maximum catechin quality index of 621.98 in the leavesof the fertilized tea plants. Similar among treatments, the catechin astringentindex of the tea increased, ranging from 2.67% to 12.63%, with increasing Nreplacement rate (P> 0.05). The mass fraction of ester catechins intea rose with the increased replacement to 4.27%, which was 10.62% higher thanthat of treatment 6, but no significant differences among the treatments (P>0.05). The changes on 7 biochemical indicators including water extract, teapolyphenols, theobromine, catechins, and amino acids in the tea leaves variedby the treatments. It indicated that the substitution of N with the mushroomwaste did not yet show a consistent long-term effect on the plant metabolism inthe duration of the experiment.