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寿宁长叶1号等茶树新品系儿茶素和氨基酸组分分析

Catechinsand Amino Acids in Shouningchangye No. 1 and Other New Tea Varieties

    摘要
  • 摘要: 以寿宁长叶1号等5个茶树新品系为供试材料，以‘福鼎大白茶’为对照材料，采用高效液相色谱法（HPLC）和超高效液相色谱-串联质谱法（UPLC-MS/MS）测定其儿茶素及游离氨基酸等组分含量，结果表明：供试茶树新品系的主要品质成分含量差异显著（P＜0.05），如茶氨酸、表没食子儿茶素-3-O-（3-O-甲基）没食子酸酯（EGCG3"Me）等。儿茶素总量为83.12～150.87 mg·g-1；游离氨基酸总量为26.40～56.22 mg·g-1，其中茶氨酸含量为15.43～41.71 mg·g-1。5份茶树品系中寿宁长叶1号、芎坑大白1号和寿宁长叶2号为高茶氨酸资源，茶氨酸含量分别为36.90 mg·g-1、40.78 mg·g-1、41.71 mg·g-1；伏际大茶树和芎坑大白1号的EGCG3"Me含量较高，分别为6.49 mg·g-1和5.88 mg·g-1。以上结果可为优质茶树品种的选育及利用提供参考。

     

    Abstract: Five newtea varieties, including Shouningchangye No. 1, and the referenceFudingdabaicha were analyzed for their catechins and free amino acids contentsby HPLC and ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS). Significant differences in the key quality constituents, such astheanine and epigallocatechin-3-O-(3-O-methyl) gallate (EGCG3"Me), werefound among the varieties (P＜0.05). The total catechin content ranged between 83.12mg·g-1 and 150.87 mg·g-1, free amino acids between 26.40 mg·g-1 and 56.22 mg·g-1, and theanine between 15.43 mg·g-1 and41.71 mg·g-1. Of the tested cultivars, Shouningchangye No. 1 washigh on theanine at 36.90 mg·g-1, Xiongkengdabai No. 1 at 40.78 mg·g-1,and Shouningchangye No. 2 at 41.71 mg·g-1; whereas Fujidachashu andXiongkengdabai No. 1 high on EGCG3"Me at 6.49 mg·g-1 and 5.88 mg·g-1,respectively. The information would be of value in breeding and utilization ofhigh quality tea varieties.

     

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