Abstract: As one of the major pathways of epigenetic regulation, DNA methylation can modify genomic DNA and take part in responding to the environmental stresses of the plants. The process requires the involvement of DNA methyltransferase. It was found, in our previous study, to occur during the cold acclimation. In this study, a full-length cDNA sequence of CsDRM2 gene was obtained using the RACE technique. Subsequently, it was submitted to NCBI and assigned an accession number of KR057963. The cDNA length of CsDRM2 was 2,328 bp containing a 1,815 bp open reading frame and the encoding 604 amino acid residues. The predicted molecular weight was 67.39 kD and theoretical isoelectric point PI 4.85. The comparison on their sequences showed a similarity greater than 65% between CsDRM2 and other reported DRMs. The phylogenetic tree analysis on CsDRM2 indicated its close genetic relationships with Sesamum indicum and Nicotiana tabacum. The qRT-PCR results on CsDRM2 revealed differential expressions of the gene at different stages of the cold acclimation. It suggested that CsDRM2 participated in the DNA methylation of the tea plant in response to cold stress.