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不同茶树品种土壤细菌群落结构和多样性特征

Community Structure and Diversity Analysis of Rhizosphere Bacteria in Different Varieties of Tea Plants

  • 摘要: 为明确不同茶树品种的土壤细菌群落结构和多样性特征,基于Illumina高通量测序技术对来源同一地块的10个茶树品种土壤细菌16S rRNA进行测序和生物信息学分析。结果表明,不同品种间细菌群落组成相似,共有优势菌门为变形菌门(Proteobacteria)、酸杆菌门(Acidobacteriota)、绿弯菌门(Chloroflexi)、放线菌门(Actinobacteriota);属水平上较高丰度的优势菌属为酸杆菌属(Acidobacteriales)。品种间的差异表现在种群丰度上,酸杆菌属(Acidobacteriales)是‘尖波黄’(JBH)、‘毛蟹’(MX)、‘蜀永1号’(SY1)、‘白叶1号’(BY1)品种的主要优势菌属,‘政和大白茶’(ZHDBC)的优势菌属为罗河杆菌属(10.04%),‘佛手’(FS)的优势菌属为JG30-KF-AS9(7.51%)。多样性分析显示‘中茶302’(ZC302)与其他9个品种的群落差异较大,其丰度和多样性指数均为最低。

     

    Abstract: Composition and diversity ofthe rhizosphere bacterial community in plantation soils of 10 different teavarieties from a same plot were studied with illumina high-throughput 16S rRNAsequencing and bioinformatic analysis. The results showed the dominant phyla inthe various rhizosphere communities with basically a similar structure to beProteobacteria, Acidobacteriota, Chloroflexi, and Actinobacteriota, while Acidobacteriales predominant inabundance at the genus level. In the soils associated with different teacultivars, the differences among the communities lied mainly in the populationabundance. For instance, Acidobacteriales was the main genus associated with the cultivar ‘Jian bohuang’ (JBH), ‘Maoxie’ (MX),‘Shuyong No 1’ (SY1), and ‘Baiye No 1’ (BY1), Rohee with ‘Zhenghe dabaicha’ (ZHDBC) at 10.04%, and JG30-KF-AS9with ‘Fo shou’ (FS) at 7.51%. The bacterial diversity at the community on the‘Zhongcha No 302’ (ZC302) lot was significantly different from those on thelots planted with the other 9 cultivars, and the abundance and diversityindices the lowest of all.

     

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