Abstract: SSR markers were used to determine the genetic diversity of the teas, Fuyun half-sib lines, from Fujian. Eighteen pairs of SSR primers were applied for PCR amplification on 13 cultivars. There were 108 bands obtained, with 67.59% of polymorphisme. The genetic similarity coefficients of half-sib lines ranged from 0.75 to 0.91, while those of controls were between 0.68 and 0.97. Controls were richer in genetic diversity than the half-sib lines. The cluster of the 13 cultivars indicated onlya remote relationship with 3 controls, including Zaochunhao. The genetic distances among the 3 controls were also too large to be clustered in a same group. The remaining 10 cultivars were clustered into two groups, 5 half-sib lines in Group I and the female parent, Fuding dabaicha, with other 4 controls in Group II. The clustering results further confirmed a greater genetic diversity of controls as compared to the half-sib lines, and significant correlations between the genetic distance and the geographical separation, parental origin, and characteristics of the teas. The results provided information for the marker-assisted selection, cross-breeding, and genetic improvement on the tea, as well as a guide for tea cultivar selection and new variety introduction.