Abstract: Effect of the application of a green manure of legume waste on the organic carbon mineralization in soil (SOCM) of tea plantations was studied by using a simulated cultivation in the laboratory. Red soil with the fertilization was incubated to observe the organic carbon mineralization dynamics, which was found to fit a first-order kinetic model. The rate of SOCM decreased rapidly initially, then continued to decline slowly in the middle of the incubation, and stabilized at the final stage. Compared to the treatments without fertilization or with a chemical fertilizer, the application of the green manure improved the microbial activity in the soil, and increased the average SOCM by 2.8% to 110.3%. However, no significant increase was observed by increasing the amount of the green manure on the proportion of the potential carbon mineralization to the total organic carbon. It was concluded that application of the green manure could increase the accumulation of organic carbon in soil at tea plantations.